Explore papers, theses, and posters that use Pioreactor.
mSystems
Four Pioreactor 20 mL chemostats maintained continuous co-cultures of host bacteria and their episymbionts. The cultures allowed researchers to follow host population collapse, adaptation, and recovery over time.
Biochemistry and Molecular Biology Education
Undergraduates used Pioreactor 20 mL systems to calibrate equipment, grow E. coli, and investigate batch and continuous cultures. The study examined how sequencing equipment training before biological experiments supported learning.
ACS Synthetic Biology
Pioreactor batch cultures and turbidostats tracked how Pseudomonas putida responded to CRISPRi gene knockdowns. Continuous cultivation supported repeated sampling for metabolomics, proteomics, and genetic analysis.
Engineering in Life Sciences
Modified Pioreactors incorporated electrodes for electrochemical cultivation of Cupriavidus necator. The platform also supported adaptive laboratory evolution under increasing sodium sulphate concentrations.
Analytical Chemistry
Pioreactors grew five bacterial species on different carbon sources to provide samples for intracellular metabolite measurements. The cultures supported development and validation of a mass-spectrometry workflow for comparing bacterial metabolism.
Nature
Pioreactor turbidostats monitored yeast regrowth after drying and maintained recovering cells for plasmid-library extraction. These experiments helped assess whether designed disordered proteins protected cells from desiccation.
bioRxiv
Pioreactor turbidostats maintained engineered E. coli populations during a five-week adaptive evolution campaign, with parallel control cultures. The experiments selected mutations that improved growth linked to Rubisco oxygenation activity.
Cell Systems
Pioreactor batch cultures provided Pseudomonas putida samples for metabolomics while recording cell density and growth rates. Comparing strains grown on glucose or acetate helped investigate how transhydrogenases maintain cellular redox and energy balance.
Food Science and Processing
A Pioreactor provided controlled temperature and stirring for yeast cultures grown on treated PLA printing waste. The study measured yeast biomass production as an early investigation of the material as a fermentation feedstock.
Nucleic Acids Research
Pioreactor 20 mL vessels housed sponge-supported Pseudomonas putida biofilms for chemical production experiments. Researchers switched from batch growth to continuous feeding to evaluate the engineered gene-expression system.
bioRxiv
A pressure-sensor plugin extended Pioreactor measurements to track gas exchange alongside optical backscatter in sealed cultures. The researchers used the system to distinguish metabolic phases in yeast growth and thermophilic syngas fermentation.
bioRxiv
The AutoGrowth application analyzed optical-density and dilution-event data from Pioreactor turbidostat experiments. The study demonstrates filtering noisy measurements, identifying growth intervals, and inspecting fitted growth curves through an interactive analysis workflow.
bioRxiv
Pioreactor 40 mL turbidostats maintained E. coli cultures at constant density across four growth media. The cultures supported measurements of growth and cell-cycle behaviour while researchers varied expression of the DNA-replication initiator DnaA.
ACS Infectious Diseases
Pioreactors recorded growth of Mycobacterium smegmatis cultures after different rifampicin exposures. The experiments tested whether prior exposure to sublethal antibiotic levels altered subsequent growth under higher drug concentrations.
Technical University of Denmark, BRIGHT
Pioreactor turbidostats supported prolonged CRISPRi experiments in Pseudomonas putida and adaptive evolution of Cupriavidus necator. Eight continuous cultures ran for 33 days to select improved growth on formate after chemical mutagenesis.
Technical University of Denmark, BRIGHT
Pioreactor turbidostats supplied steady-state cultures for comparing Cupriavidus necator strains, tracing metabolic fluxes, and studying responses to changing cultivation conditions. These experiments applied LC-MS metabolomics to investigate the organism’s metabolic flexibility.
e-Conference on Innovation in Microbial Control 2025
A custom morbidostat algorithm controlled a Pioreactor culture of Staphylococcus aureus under bacitracin exposure for three days. The conference abstract reports an increase in the minimum inhibitory concentration during this adaptive evolution experiment.
2025 International Conference on Chemical Engineering and Biological Science (CEBS 2025)
Pioreactor cultures provided controlled temperatures for E. coli producing recombinant GFP. The researcher varied induction and medium conditions, then measured protein yield and solubility to fit a response-surface model.
32nd International Conference on Yeast Genetics and Molecular Biology
A modified Pioreactor combined continuous cultivation with a miniature UV mutagenesis module, revised pumping, and a sampling port. The conference abstract reports testing the system with seven yeast species for adaptive laboratory evolution.
29th International Conference on Optical Fiber Sensors (OFS 2025), Proceedings of SPIE
A redesigned Pioreactor coupled excitation LEDs and a spectrometer to the culture vial through optical fibers. Cyanobacterial experiments compared fluorescence and optical-density measurements during excessive illumination and culture contamination.
Biofilms 11
Pioreactors housed sludge communities enriched in glycogen-accumulating organisms while researchers tested phage cocktails. The poster abstract reports restored phosphorus removal after targeting organisms that interfere with wastewater treatment.
Biosensors
Researchers adapted a Pioreactor with excitation LEDs, optical fibers, and a spectrometer to monitor cyanobacterial fluorescence. A software plugin coordinated spectral measurements with the existing culture-control functions.
Technical University of Denmark, DTU Bioengineering
Pioreactor turbidostats supported adaptive laboratory evolution of Pseudomonas putida for tolerance to deep eutectic solvents used in lignin processing. The thesis compared these continuous-culture populations with strains evolved through tube-based batch transfers.
bioRxiv
Pioreactor 20 mL vessels maintained heated and stirred reactions for enzymatic degradation of post-consumer PET plastic. Researchers compared engineered PETases and tracked breakdown products over 72 hours.
University of Cantabria
This thesis modified a Pioreactor with improved lighting and modules for fluorescence and Raman measurements. Optical measurements tested the new readouts, while contamination limited the culture-growth experiments.
University of Washington, School of Oceanography
Three Pioreactors incubated natural microbial communities from the equatorial Pacific. The experiments compared continuous and batch incubation to investigate community responses to added inorganic nutrients.
University of New Hampshire, Department of Chemistry
Pioreactor cultures supported small-scale E. coli growth experiments and plasmid DNA measurements. The poster presents these experiments as groundwork for investigating temperature-dependent DNA yields before moving to a larger bioreactor.
